Reproducibility is key when it comes to pharmaceutical research. Tanso Biosciences' GPCR functional assay is not only sensitive and versatile but highly reproducible. We have conducted a series of experiments to verify the consistency of our assay, and the results are impressive.
Experiment 1
We performed concentration-response analyses for β1 adrenoceptor (ADRB1) vs. isoproterenol on three different days (Figure 1, Table 1). Each test yielded a very close EC50 value, indicating excellent reproducibility of the assay.
This figure displays the results of three independent tests conducted on different days, in which ADRB1-expressing reporter cells were treated with the receptor agonist isoproterenol (n = 4, mean ± SEM). The data were fitted to sigmoid curves with a common Hill coefficient.
| Test | EC50 | Hill coefficient |
| 1 | 33 nM | 2.8 |
| 2 | 36 nM | 2.8 |
| 3 | 31 nM | 2.8 |
| Aggregate | 33 nM | 2.8 |
Experiment 2
We examined the variability between experimenters. Two experimenters in our lab conducted concentration-response analyses for type 1 angiotensin 2 receptor (AGTR1) and yielded comparable results both in the agonist mode (Figure 2, Table 2) and the antagonist mode (Figure 3, Table 3).
This figure displays the results of identical tests conducted by two experimenters, in which AGTR1-expressing reporter cells were treated with the receptor agonist angiotensin II (n = 4, mean ± SEM). The data were fitted to sigmoid curves with a common Hill coefficient.
| Experimenter | EC50 | Hill coefficient |
| 1 | 160 pM | 2.0 |
| 2 | 120 pM | 2.0 |
| Aggregate | 140 pM | 2.0 |
This figure displays the results of identical tests conducted by two experimenters, in which AGTR1-expressing reporter cells were treated with the receptor antagonist valsartan in the presence of angiotensin II (n = 4, mean ± SEM). The data were fitted to sigmoid curves with a common Hill coefficient.
| Experimenter | IC50 | Hill coefficient |
| 1 | 2.1 nM | -1.4 |
| 2 | 2.4 nM | -1.4 |
| Aggregate | 2.2 nM | -1.4 |
Conclusion
Overall, our verification process demonstrated that Tanso Biosciences' GPCR functional assay consistently produces reliable results, regardless of who conducts the test or when it is conducted. Trust in the reproducibility of our assay and choose Tanso Biosciences for your research needs.
