Human C5AR1

Figure 1. Concentration-dependent activation of C5AR1 by C5a

Reporter cells were transfected with either the expression plasmid for human complement C5a receptor 1 (C5AR1) or the mock plasmid and treated with various concentrations of C5a. Data points shown are the mean ± SEM of an experiment (n = 3), and the curve is a fit to Hill equation with an EC50 of 220 pM.

Figure 2. Concentration-dependent inhibition of C5AR1

Reporter cells were transfected with the expression plasmid for human C5AR1 and treated with various concentrations of an antagonist in the presence of C5a. Data points shown are the mean ± SEM of an experiment (n = 3).