Human NPFFR1

Figure. Concentration-dependent activation of NPFFR1 by NPFF

Reporter cells were transfected with either the expression plasmid for human neuropeptide FF receptor 1 (NPFFR1) or the mock plasmid and treated with various concentrations of NPFF. Data points shown are the mean ± SEM of an experiment (n = 3), and the curve is a fit to Hill equation with an EC50 of 22 nM.